Chủ Nhật, 14 tháng 1, 2018

Journal club Chalmers note - Một vài công cụ trong sinh học

Question:
  • What do you use to find the newest literature and stay up to date in your field? y
  • Can you recommend a neat program to generate good-looking protein domain structures?
  • A particularly user-friendly primer design app?
  • A clever way to make taking notes more efficient?  
  • A website to draw pretty phylogenetic trees?
  • Endnote, Mendeley, or something completely different?
  • Have you found a great online to-do list generator?
  • Etc

1.     Bibliography update

I think the principle here is what is your subject and it could be in which database, from this you can use any tool to find right data and have a good materials.
The second things is your key work and search tools, if it right and correct (for example GH61 is AA9 and what is your database, focus on gene or focus on process -> show you the results search much more precise.
The last things is how you treat your biblio, become a good analysis of quantitative and qualitative.

1.1.  Database

1.1.1.      What do you use to find the newest literature and stay up to date in your field?

To build a chart trend line wit query
Scholar plot
or other field Google trends

1.1.2.      Google scholar

Easy to use alert service for newly published research. Define your own queries (e.g. keywords that appear in the title/abstract, author names, etc.).
(Note: Google Scholar has a similar service, so do many other websites, like Scopus, etc. Pick your favourite!)
Research gate
Subscribe new paper form author

1.2.  Citation and manage

1.2.1.      Endnote

1.2.2.      Mendeley

1.2.3.      Zotero

2.     Text edit

2.1.  ShareLatex

2.2.   Nopad++

2.3.   A clever way to make taking notes more efficient? 

3.     Data analysis

IBS: Illustrator for biological sequences (http://ibs.biocuckoo.org/)
Nifty program to generate nucleotide or protein domain structures. Very easy to use, freeware (Windows/Mac/Linux). Allows graphics export as SVG, PDF and EPS, so you can very easily polish the figures in Illustrator.
Costs 10$, but it’s SO good for Mac to easily switch between your 20 open Excel files, etc.
iTOL (interactive tree of life) (http://itol.embl.de/)
Makes beautiful and customisable phylogenetic trees. Input multiple sequence alignment.

Very easy to use shading tool for multiple sequence alignments.
Website where you can easily create your own customised background noise soundscape to improve concentration: fire crackling, rain, train noises, white noise. Very good if you can’t work to music, but the environment is too distracting.
Best to-do list I have found online. You can make a new list for each project and keep track of upcoming tasks. Colour-tag or set due dates. You can even invite more users for a collaborative to-do list.

3.1.  Gene

3.1.1.      A particularly user-friendly primer design app?

Generate nice sequence alignment (different shading possible), add PDB entries to the alignment to get information about secondary protein structures.

Biological sequence alignment editor and viewing tool for Windows. Trimming, shading, etc. possible.

3.1.2.      A website to draw pretty phylogenetic trees?

3.2.  Protein

3.2.1.      Can you recommend a neat program to generate good-looking protein domain structures?

Ø  PyMOL
Make 3D structure
+ can write command
+ make a video
+ find ligand and protein 
Freeware program (both Mac and Windows) for visualising protein and molecule structures. You can get figures with very high quality. Extensive wiki available. It’s possible to make videos/gifs of molecules (e.g. zooming into the active site). Current users: Vero, Johan, Scott, Jae.


Cazy pedia
Find the gene classify enzyme

Fugalgenomic.ca
Find sequencing of group enzyme



3.3.  Data

3.3.1.      MatLab

Matlab (which is available through a Chalmers licence) also offers many apps for various applications that have a graphical user interface and don’t require programming experience.
For example:
·         Curve fitting app: e.g. curve fitting for kinetic data, contour plots (3D)
·         Distribution fitting tool
·         Computational biology (sequence alignment, phylogenetic tree)
·         For modelling of metabolic pathways. You draw pictures of e.g. signalling pathway and put your data in à get model estimates. Both Windows and Mac.
·         Webbased tool to draw (simple) molecules.
Notepad++
·         Text editor for windows. Very good for programming, because it recognises different programming languages and aligns the code properly when you open a file
·         Screen capture tool that can capture both static images and videos. It’s possible to even annotate the captured image.
·         (Note: Windows 10 has Snipping Tool included that does targeted screenshots, and for Mac users Cmd+shift+4 is very useful when you want to make a screenshot of a targeted region on your screen)

3.3.2.      Excel

Corrects typos and grammer mistakes not only in Word, but also in Gmail, Facebook and everywhere else on the web.

Inline Charts (Sparklines) in Excel
It’s possible to make little inline charts in Excel to see a trend at a quick glance.
Windows:

Mac:

Result:

(Note: Excel has A LOT more functionalities than we usually use. For example under Data>Data Analysis, many statistical tests can be done, e.g. 2-factor ANOVA, correlation, fourier analysis, regression, random number generator, t-tests, etc.)

4.     Store and sharing

4.1.  Have you found a great online to-do list generator?

-          Google calendar

-           

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